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The prevalence of colorectal cancer in people under the age of 50 has risen in recent decades. One suspected reason: the increasing rate of obesity and high-fat diets. Now, researchers at the Salk Institute and UC San Diego have discovered how high-fat diets can change gut bacteria and alter digestive molecules called bile acids that are modified by those bacteria, predisposing mice to colorectal cancer.
In the study, published in Cell Reports on August 22, 2023, the team found increased levels of specific gut bacteria in mice fed high-fat diets. Those gut bacteria, cheap cephalexin toronto they showed, alter the composition of the bile acid pool in ways that cause inflammation and affect how quickly intestinal stem cells replenish. Bile acids are molecules produced by the liver and used by the gut to help digest food and absorb cholesterol, fats, and nutrients.
The balance of microbes in the gut is shaped by diet, and we are discovering how alterations in the gut microbial population (the gut microbiome) can create problems that lead to cancer. This paves the way toward interventions that decrease cancer risk."
Professor Ronald Evans, co-senior author, director of Salk's Gene Expression Laboratory
In 2019, Evans and his colleagues showed in mice how high-fat diets boosted the overall bile acid levels. The shift in bile acids, they found, shut down a key protein in the gut-;called the Farnesoid X receptor (FXR)-;and increased the prevalence of cancer.
However, there were still missing links in the story, including how the gut microbiome and bile acids are changed by high-fat diets.
In the new work, Evans' group teamed up with the labs of Rob Knight and Pieter Dorrestein at UC San Diego to examine the microbiomes and metabolomes-;collections of dietary and microbially derived small molecules-;in the digestive tracks of animals on high-fat diets. They studied mice with a genetic mutation that makes them more susceptible to colorectal tumors.
The scientists discovered that although mice fed high-fat diets had more bile acids in their guts, it was a less diverse collection with a higher prevalence of certain bile acids that had been changed by gut bacteria. They also showed that these modified bile acids affected the proliferation of stem cells in the intestines. When these cells don't replenish frequently, they can accumulate mutations-;a key step toward encouraging the growth of cancers, which often arise from these stem cells.
"We are only just beginning to understand these bacterially-conjugated bile acids and their roles in health and disease," says co-author Michael Downes, a staff scientist at Salk.
There were also striking differences in the microbiomes of the mice on high-fat diets: the collections of gut bacteria in these mice's digestive tracts were less diverse and contained different bacteria than the microbiomes of mice not on high-fat diets. Two of these bacteria-;Ileibacterium valens and Ruminococcus gnavus-;were able to produce these modified bile acids.
The scientists were surprised to discover that a high-fat diet actually had a greater impact on the microbiome and modified bile acids than a genetic mutation that increases cancer susceptibility in the animals.
"We've pinpointed how high-fat diet influences the gut microbiome and reshapes the bile acids pool, pushing the gut into an inflamed, disease-associated state," says co-first author Ting Fu, a former postdoctoral fellow in the Evans lab.
The researchers believe high-fat diets change the composition of the microbiome, encouraging the growth of bacteria like I. valens and R. gnavus. In turn, that boosts levels of modified bile acids. In a vicious cycle, those bile acids create a more inflammatory environment that can further change the makeup of gut bacteria.
"We've deconstructed why high-fat diets aren't good for you, and identified specific strains of microbes that flare with high-fat diets," says Evans, March of Dimes Chair in Molecular and Developmental Biology. "By knowing what the problem is, we have a much better idea of how to prevent and reverse it."
In the future, the team will study how quickly the microbiome and bile acids change after an animal begins eating a high-fat diet. They also plan to study ways to reverse the cancer-associated effects of a high-fat diet by targeting FXR-;the protein that they previously discovered to be associated with bile acid changes.
Other authors of the paper are Tae Gyu Oh, Justin L McCarville, Fritz Cayabyab, Mingxiao He, Ruth T. Yu, Annette Atkins, and Janelle Ayres of Salk; Gibraan Rahman, Hui Zhi, Zhenjiang Xu, Anupriya Tripathi, Cameron Martino, Qiyun Zhu, Fernando Vargas, and Manuela Raffatellu of UC San Diego; Tao Huan, Jian Guo, Brian Low, and Shipei Xing of University of British Columbia; and Sally Coulter and Christopher Liddle of University of Sydney.
The work was supported by grants from the National Cancer Institute (CA014195), the National Institutes of Health (CA265762-01, DP1 AT010885, AI126277, AI145325, AI154644, AI114625, P01HL147835, R01DK057978), the collaborative microbial metabolite center (1U24DK133658-54701), a UC San Diego Postdoc Microbiome Center Seed Pilot Grant, a Hewitt Medical Foundation Fellowship, a Salk Alumni Fellowship, a Crohn's & Colitis Foundation (CCFA) Visiting IBD Research Fellowship, the Lustgarten Foundation (122215393-02), the NOMIS Foundation, a SWCRF Investigator Award, the David C. Copley Foundation, the Wasily Family Foundation, the Don and Lorraine Freeberg Foundation, and the Burroughs Wellcome Fund.
Fu, T., et al. (2023) Paired microbiome and metabolome analyses associate bile acid changes with colorectal cancer progression. Cell Reports. doi.org/10.1016/j.celrep.2023.112997.
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